Enzymes and Inhibitors
Sigma Aldrich Fine Chemicals Biosciences DNase I recombinant, RNase-free, 10000 units
DNase I recombinant, RNase-free may be used to degrade DNA in applications that are sensitive to the presence of Rnase.
New England Biolabs, Inc. T4 Polynucleotide Kinase – 500 units
T4 PNK catalyzes the transfer and exchange of P from the gamma; position of ATP to the 5 -hydroxyl terminus of polynucleotides (double-and single-stranded DNA and RNA) and nucleoside 3-monophosphates. Polynucleotide Kinase also catalyzes the removal of 3-phosphoryl groups from 3-phosphoryl polynucleotides, deoxynucleoside 3-monophosphates and deoxynucleoside 3-diphosphates.
- 5' phosphorylation of DNA/RNA for subsequent ligation
- End labeling DNA or RNA for probes and DNA sequencing
- Removal of 3' phosphoryl groups
New England Biolabs, Inc. T4 DNA Ligase – 100,000 units – 400,000 units/ml
The industry standard for performance and quality. T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5' phosphate and 3' hydroxyl termini in duplex DNA or RNA.
- Catalyzes the formation of a phosphodiester bond between juxtaposed 5' phosphate and 3' hydroxyl termini in duplex DNA or RNA
- Joins blunt and cohesive end termini as well as repair single stranded nicks in duplex DNA
- T4 DNA Ligase variants: Salt-T4, Hi-T4, Instant Sticky-end Ligase MM, Blunt/TA Ligase MM
New England Biolabs, Inc. T4 DNA Ligase – 20,000 units – 2,000,000 units/ml
The industry standard for performance and quality. T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5' phosphate and 3' hydroxyl termini in duplex DNA or RNA.
- Catalyzes the formation of a phosphodiester bond between juxtaposed 5' phosphate and 3' hydroxyl termini in duplex DNA or RNA
- Joins blunt and cohesive end termini as well as repair single stranded nicks in duplex DNA
- T4 DNA Ligase variants: Salt-T4, Hi-T4, Instant Sticky-end Ligase MM, Blunt/TA Ligase MM
New England Biolabs, Inc. MspI – 5000 units
A restriction endonuclease that recognizes the sequence C^CG_G.
New England Biolabs, Inc. NdeI – 4000 units
A restriction endonuclease that recognizes the sequence CA^TA_TG.
New England Biolabs, Inc. HindIII – 50000 units
A restriction endonuclease that recognizes the sequence A^AGCT_T.
New England Biolabs, Inc. FspI – 500 units
A restriction endonuclease that recognizes the sequence TGC_^GCA.
New England Biolabs, Inc. SalI – 10000 units
A restriction endonuclease that recognizes the sequence G^TCGA_C.
New England Biolabs, Inc. XmnI – 1000 units
A restriction endonuclease that recognizes the sequence GAANN_^NNTTC.
New England Biolabs, Inc. XhoI – 25000 units
A restriction endonuclease that recognizes the sequence C^TCGA_G.
New England Biolabs, Inc. XmaI – 500 units
A restriction endonuclease that recognizes the sequence C^CCGG_G.
New England Biolabs, Inc. AflII – 2000 units
A restriction endonuclease that recognizes the sequence C^TTAA_G.